Gene Ontology (GO) annotations related to this gene include mRNA binding. The human NOS2 promoter contains multiple potential binding sites for transcription factors including binding sites for NFjB, activator protein 1 (AP-1), CCAAT/enhancer-binding protein (C/EBP β) … The results showed that P. gingivalis coated with anti -P. gingivalis antibody before co-culture with neuron cells could decrease the gene expression of TNF , IL1B , and NOS2 of neuron cells. Recently, NOS activity has been demonstrated in several bacterial species, including notorious pathogens Bacillus anthracis and Staphylococcus aureus. Nitric oxide synthase-2 (NOS2) is a key gene that displays species-specific outcomes via altered regulation of the gene promoter and via post-transcriptional mechanisms in humans that are not found in mice. The gene expressions of the TNF , IL1B , NOS2 in neurons was evaluated using RT-qPCR. (from RefSeq NM_000625) RefSeq Summary (NM_000625): Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human NOS2 polyclonal antibody (dilution ratio 1:1000) at 4℃ overnight. The NOS2 antibody has been validated for the following applications: IHC, WB, ELISA analysis. Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Activated mouse macrophages metabolize arginine via NO synthase (NOS2) to produce NO as an antimicrobial effector. NANOS2 (Nanos C2HC-Type Zinc Finger 2) is a Protein Coding gene. The OD value is proportional to the concentration of Human NOS2/iNOS. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S … This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. The resulting levels of NO produced by activation of human NOS2 are different from the levels of NO produced by mouse Nos2. Diseases associated with NANOS2 include Spermatocytoma and Spermatogenic Failure, X-Linked, 1. The NOS2 gene is highly expressed in liver and is inducible by a combination of bacterial endotoxins and certain cytokines, including IL-1, IFNγ and TNFα. Johannesen et al. ACKNOWLEDGMENTS. The enzyme nitric oxide synthase 2 (NOS2), often called inducible NOS, plays a central role in the inflammatory reactions that follow infection or tissue damage. It helps in immune defense against pathogens by producing nitric oxide. Structurally, the protein is reported to be 131.1 kilodaltons in mass. This work was supported by the Wellcome Trust and The Medical Research Council. View mouse Nos2 Chr11:78920787-78960226 with: phenotypes, sequences, polymorphisms, proteins, references, function, expression Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). HEP-NOS, iNOS, NOS, NOS2A. It displays high affinity for Ca 2+ /calmodulin. Quality is guaranteed. Homo sapiens (human) : NOS2 (nitric oxide synthase 2) HGNC Alliance Mus musculus (house mouse) : Nos2 (nitric oxide synthase 2, inducible) MGI Alliance Chinchilla lanigera (long-tailed chinchilla) : Nos2 (nitric oxide synthase 2) Pan paniscus (bonobo/pygmy chimpanzee) : NOS2 (nitric oxide synthase 2) NOS2 Uniprot Gene Name NITRIC OXIDE SYNTHASE, INDUCIBLE Uniprot Protein Name ENSG00000007171 Ensembl Gene Id anti-NOS2 Antibody reacting with Rabbit and identified with WB, IHC, IF, ELISA. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. This model demonstrates an altered immune redox state reminiscent of the human disease and capitalizes on data indicating critical differences between human and mouse immune responses, particularly in nitric oxide levels produced by immune activation of the NOS2 gene. The NOS2 antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human NOS2. Contact Us | Register or Login ... NOS2 (Myc-DDK-tagged)-Human nitric oxide synthase 2, inducible (NOS2) 10 ug; USD 1,210.00. a free radical. SFARI Gene Update. This antibody recognizes human,mouse antigen. iNOS in Human Brain. NP100041. NOS2 (Nitric Oxide Synthase 2, Inducible, NOS2) Short Description: Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Product Pages: Species: Gene Names: Gene Aliases: RefSeq Accessions: SNP IDs (if applicable): Mature Names (if applicable): Hs00164883_CE details, Hs00164883_CE search: Human Genetic association has been found between the NOS2 gene (formerly known as the NOS2A gene) and autism in a Korean population cohort (Kim et al., 2009). Orthologs from Annotation Pipeline: 271 organisms have orthologs with human gene NOS2; Orthologs qSTAR qPCR primer pairs against Homo sapiens gene NOS2. NOS2 Antibody (C-11) is a high quality monoclonal NOS2 antibody (also designated iNOS antibody or NOS2A antibody) suitable for the detection of … Whereas the function of Nos2 in murine host immunity is well characterized, that of human NOS2 has remained elusive and controversial. iNOS Inducible nitric oxide synthase (iNOS), along with neuronal nitric oxide synthase (nNOS) and endothelial nitric oxide synthase (eNOS), catalyze the generation of nitric oxide and L-citrulline from L-arginine and molecular oxygen. 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